Online HPLC with DirectInject-LC

Enhance Process Understanding with Online HPLC Analysis

DirectInject-LC™ transforms HPLC into an online technique for reaction analysis that enables scientists to gain the data required to understand complex processes in near real-time.

DirectInject-LC bridges the gap between offline methods and real-time, in-situ process analytical technologies (PAT), such as ReactIR™, ReactRaman™, and EasyViewer™, by effortlessly sampling a wide range of chemical reactions, with automated sample quench, dilution, and delivery to a chromatography instrument.

Combine DirectInject-LC with world-leading reaction analysis and modeling software to gain near real-time insight into quantitative reaction concentrations and kinetics, crystallization processes, and impurity profiles.

Discover online HPLC analysis solutions.

HPLC for Reaction Analysis

With DirectInject-LC, HPLC can now be used for near real-time reaction and crystallization understanding. Fully automated rapid reaction sampling and injection transforms HPLC into a powerful new technology for online reaction monitoring.

Analysis of the data with world-leading iC Software, specifically designed for reaction analysis and modeling, enables deeper reaction insight and accelerated process and product development.

Effortless In-Situ Sampling

Hands-free and reproducible reaction sampling, preparation, and injection with the EasySampler Probe and Flow Sampler. Continuously collect representative samples, quenched at reaction conditions, with proven EasySampler™ technology that enables analysis of complex, multiphase, and challenging chemistry.

EasyFrit allows selective solution phase sampling with a self-cleaning filter
Flow Sampler Module precisely samples from continuous flow reactions

real time liquid chromatography software

iC LC Software

Built on the iC platform for easy integration of PAT control, analysis, and orthogonal data visualization.

Set the automated sampling interval and trigger the HPLC method execution from iC LC. Live Chromatography Data System (CDS) integration provides direct visualization and analysis of chromatograms.

View 3D surface plot and 2D chromatograms

Easily switch between HPLC detector channels
Double-click to profile a peak
Peaks from all channels displayed together

Easily transfer and integrate data to use in modeling for further understanding.

Comprehensive Reaction Monitoring and Understanding — Application Examples

Continuous Chiral Crystallization

Researchers achieved supersaturation control of a continuous chiral crystallization with high yield of the enantiopure product.

Examine the Entire Reaction Mass

A 5-fold increase in reaction rate was obtained from a mechanistic understanding of entire reaction mass, while mitigating sample decomposition.

Structurally Similar Species

Reaction understanding enabled full synthetic control of an important organic conductor during an air-sensitive cross-coupling with multiple similar intermediates.

Reaction Analysis Resources

Cai, I., Malig, T. C., Kurita, K. L., Derasp, J. S., Sirois, L. E., & Hein, J. E. (2024). Investigating the Origin of Epimerization Attenuation during Pd-Catalyzed Cross-Coupling Reactions. ACS Catalysis, 14(16), 12331–12341. https://doi.org/10.1021/acscatal.4c03401
Shi, Y., Derasp, J. S., Guzman, S. M., Patrick, B. O., & Hein, J. E. (2024). Halide salts alleviate TMSOK inhibition in Suzuki–Miyaura Cross-Couplings. ACS Catalysis, 14(16), 12671–12680. https://doi.org/10.1021/acscatal.4c02407
Kukor, A. J., St-Jean, F., Stumpf, A., Malig, T. C., Piechowicz, K. A., Kurita, K., & Hein, J. E. (2023). Guided optimization of a crystallization-induced diastereomer transformation to access a key navoximod intermediate. Reaction Chemistry & Engineering, 8(6), 1294–1299. https://doi.org/10.1039/d3re00077j
Deem, M. C., Derasp, J. S., Malig, T. C., Legard, K., Berlinguette, C. P., & Hein, J. E. (2022). Ring walking as a regioselectivity control element in Pd-catalyzed C-N cross-coupling. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-30255-1
Kukor, A. J., Depner, N., Cai, I., Tucker, J. L., Culhane, J. C., & Hein, J. E. (2022). Enantioselective synthesis of (−)-tetrabenazine via continuous crystallization-induced diastereomer transformation. Chemical Science, 13(36), 10765–10772. https://doi.org/10.1039/d2sc01825j
Malig, T. C., Kumar, A., & Kurita, K. L. (2022). Online and in situ monitoring of the exchange, transmetalation, and Cross-Coupling of a Negishi reaction. Organic Process Research & Development, 26(5), 1514–1519. https://doi.org/10.1021/acs.oprd.2c00081
Christensen, M., Yunker, L. P. E., Shiri, P., Zepel, T., Prieto, P. L., Grunert, S., Bork, F., & Hein, J. E. (2021). Automation isn’t automatic. Chemical Science, 12(47), 15473–15490. https://doi.org/10.1039/d1sc04588a
Sato, Y., Liu, J., Kukor, A. J., Culhane, J. C., Tucker, J. L., Kucera, D. J., Cochran, B. M., & Hein, J. E. (2021). Real-Time monitoring of Solid–Liquid slurries: Optimized synthesis of tetrabenazine. The Journal of Organic Chemistry, 86(20), 14069–14078. https://doi.org/10.1021/acs.joc.1c01098
Kukor, A. J., Guy, M. A., Hawkins, J. M., & Hein, J. E. (2021). A robust new tool for online solution-phase sampling of crystallizations. Reaction Chemistry & Engineering, 6(11), 2042–2049. https://doi.org/10.1039/d1re00284h
Malig, T. C., Yunker, L. P. E., Steiner, S., & Hein, J. E. (2020). Online HPLC Analysis of Buchwald-Hartwig Aminations from Within an Inert Environment. Analytical Chemistry. https://doi.org/10.26434/chemrxiv.12798083.v1
Rougeot, C., Situ, H., Cao, B. H., Vlachos, V., & Hein, J. E. (2017). Automated reaction progress monitoring of heterogeneous reactions: crystallization-induced stereoselectivity in amine-catalyzed aldol reactions. Reaction Chemistry & Engineering, 2(2), 226–231. https://doi.org/10.1039/c6re00211k

МЕТТЛЕР ТОЛЕДО рекомендует

Online HPLC with DirectInject-LC

Enhance Process Understanding with Online HPLC Analysis

HPLC for Reaction Analysis

Effortless In-Situ Sampling

iC LC Software