Kinetic Modelling of Oligonucleotide Detritylation
High Throughput Experimentation
Program Overview
- Use of kinetic modeling with oligonucleotides.
- Nuances of liquid phase oligonucleotide kinetics.
- Combining high throughput experimentation with simple kinetic modeling for strategic experimentation.
This presentation will discuss two examples of using kinetic modeling to advance the development of the detritylation stage of a liquid phase oligonucleotide synthesis process. Case study 1 will explore developing a kinetic model that can cope to various changes in process parameters including nucleotide type, acid concentration and oligonucleotide concentration. Case study 2 will explore the use of kinetics and high throughput experimentation to shortlist a selection of reagents and solvents for detritylation.
Speakers
Lizzie Boulter
Senior Scientist in Chmical Development, AstraZeneca
Lizzie is currently a senior scientist in Chemical Development at AstraZeneca. She has worked there since 2021 and has a focus on oligonucleotide synthesis and processing technologies. Prior to this she completed her MChem at the University of Oxford.
