Modeling the Gibbs-Donnan Effect for High Concentration Protein TFF Process in Downstream Purification
Scale-up Suite Guest Webinar
Program Overview
- Background on Gibbs-Donnan effect and its importance during diafiltration and ultrafiltration
- Model predictions of pH and buffer concentration drift
- Optimization of starting buffer pH and buffer concentration to achieve target range
Using the Gibbs-Donnan model for high concentration protein Tangential Flow Filtration (TFF) process, including diafiltration and ultrafiltration, to predict pH drift and buffer concentration change during the process. The model fitting with lab data was implemented to predict process conditions and increase process development efficiency
Guest Presenter
Cuixian (Trisha) Yang
Protein Downstream Process Engineer, Biologics Process R&D, Merck
